生命科学
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耗材
Phos-tag™ 琼脂糖枪头
Phos-tag™ Tip

Phos-tag™ 琼脂糖枪头新品速递图wako.jpg

Phos-tag™ Tip




  可特异性捕捉磷酸基团的功能性分子“Phos-tag ™”的磷酸化肽纯化用枪头。

  枪头里含有 Phos-tag ™ 琼脂糖,是即开即用的前处理工具,适用于生理状态下低分子量磷酸化分子(核酸和多肽等)的分离和富集。




原理


把 Phos-tag™ Tip 装在注射器(购买产品附送)上使用。


                                   

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Phos-tag ™ Tip 的结构


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优点、特色


 操作时间少于30分钟 

 高回收率可重复利用

 无需昂贵仪器

 缓冲液为生理 pH 条件下





案例、应用


分离磷酸化肽使用例


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Phos-tag™ 系列

磷酸化蛋白新方法!



  Phos-tag™是一种能与磷酸离子特异性结合的功能性分子。它可用于磷酸化蛋白的分离(Phos-tag™ Acrylamide)、Western Blot 检测(Phos-tag™ Biotin)、蛋白纯化 (Phos-tag™Agarose)及质谱分析 MALDI-TOF/MS (Phos-tag™ Mass Analytical Kit)。


◆Phos-tag™ 的基本结构

Phos-tag



原理:


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特点:


●   与 -2 价磷酸根离子的亲和性和选择性高于其它阴离子

●   在 pH 5-8 的生理环境下生成稳定的复合物




相关应用:


Phos-tag应用



相关产品:


 产品名称

 用  途

 Phos-tag™ Acrylamide

 分离:SDS - PAGE 分离不同磷酸化水平的蛋白

 SuperSep Phos-tag™

 分离:预制胶中含有 50 μM Phos-tag™ Acrylamide

 Phos-tag™ Biotin

 检测:代替 Western Blot 检测中的磷酸化抗体

 Phos-tag™ Agarose

 纯化:通用柱层析,纯化磷酸化蛋白

 Phos-tag™ Mass

 Analytical Kit

 分析:用于质谱 MALDI-TOF/MS 分析,提高磷酸化分子的检测灵敏度


phos-tag™ 由日本广岛大学研究生院医齿药学综合研究科医药分子功能科学研究室开发。


更多产品信息,请点击:


Phos-tag实验指导手册 第6版 2020.jpg


Phos-tag 第6版说明书




Phos-tag 系列产品封面图.jpg


Phos-tag系列 ver. 8




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·  Phosphoinositide kinase signaling controls ER-PM cross-talk[J]. Molecular biology of the cell, 2016, 27(7): 1170-1180,Omnus D J, Manford A G, Bader J M, et al.

·  A multiple covalent crosslinked soft hydrogel for bioseparation[J]. Chemical Communications, 2016, 52(15): 3247-3250,Liu Z, Fan L, Xiao H, et al.

·  Advances in crop proteomics: PTMs of proteins under abiotic stress[J]. Proteomics, 2016, 16(5): 847-865,Wu X, Gong F, Cao D, et al.

·  Cyclin-Dependent Kinase Co-Ordinates Carbohydrate Metabolism and Cell Cycle in S. cerevisiae[J]. Molecular cell, 2016, 62(4): 546-557,Zhao G, Chen Y, Carey L, et al.

·  Carbon Monoxide Gas Is Not Inert, but Global, in Its Consequences for Bacterial Gene Expression, Iron Acquisition, and Antibiotic Resistance[J]. Antioxidants & redox signaling, 2016,Wareham L K, Begg R, Jesse H E, et al.

·  Two-layer regulation of PAQR3 on ATG14-linked class III PtdIns3K activation upon glucose starvation[J]. Autophagy, 2016: 1-2,Xu D, Wang Z, Chen Y.

·  Regulation of sphingolipid biosynthesis by the morphogenesis checkpoint kinase Swe1[J]. Journal of Biological Chemistry, 2016, 291(5): 2524-2534,Chauhan N, Han G, Somashekarappa N, et al.

·  PAX5 tyrosine phosphorylation by SYK co-operatively functions with its serine phosphorylation to cancel the PAX5-dependent repression of BLIMP1: A mechanism for antigen-triggered plasma cell differentiation[J]. Biochemical and biophysical research communications, 2016, 475(2): 176-181,Inagaki Y, Hayakawa F, Hirano D, et al.

·  A Combined Computational and Genetic Approach Uncovers Network Interactions of the Cyanobacterial Circadian Clock[J]. Journal of Bacteriology, 2016: JB. 00235-16,Boyd J S, Cheng R R, Paddock M L, et al.

·  HuR mediates motility of human bone marrow-derived mesenchymal stem cells triggered by sphingosine 1-phosphate in liver fibrosis[J]. Journal of Molecular Medicine, 2016: 1-14,Chang N, Ge J, Xiu L, et al.

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References on Phos-tag™ Chemistry



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