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Phos-tag™ 质谱分析试剂盒
Phos-tag™ Mass Analytical Kit

Phos-tag™ Mass Analytical Kit新品速递图wako.jpg

用于 MALDI-TOF/MS检测,提高检测灵敏度!


  用于质谱分析的试剂套装。

  Phos-tag Mass Analytical Kit 是用于质谱分析的试剂套装,可配套 MALDI-TOF/MS 使用。可检测磷酸化分子- Phos-tag® 复合物,通常可提高低磷酸化分子的检测灵敏度。


试剂盒内容:

● Phos-tag™ MS-101L  5 mg([C27H29N6O64Zn2]3+ MW:581.4)

● Phos-tag™ MS-101H 5 mg([C27H29N6O68Zn2]3+ MW:589.4)

● Phos-tag™ MS-101N 10 mg([C27H29N6OZn2]3+ MW:584.3)


原理:


原理


优点、特色:


● CH3COO- 等价结合在 Phos-tag™ MS-101 上。

● 在溶液中,不含有阴离子的 Phos-tag™ MS-101 带有+3价。

● 检测前需制备 1 mM 的 Phos-tag™ MS-101L,MS-101H 或者 MS-101N(溶于水)。



案例、应用:


【使用例子:检测 Phos-tag™ - 磷酸化 LPA 复合体】


01.jpg


由于正电荷增大磷酸化 LPA 检测灵敏度上升



Phos-tag™ 系列

磷酸化蛋白新方法!



  Phos-tag™ 是一种能与磷酸离子特异性结合的功能性分子。它可用于磷酸化蛋白的分离(Phos-tag™ Acrylamide)、Western Blot 检测(Phos-tag™ Biotin)、蛋白纯化 (Phos-tag™ Agarose)及质谱分析 MALDI-TOF/MS (Phos-tag™ Mass Analytical Kit)。


Phos-tag™ 的基本结构:

Phos-tag

特点:

与-2价磷酸根离子的亲和性和选择性高于其它阴离子

在 pH 5-8 的生理环境下生成稳定的复合物



原理:


1314447039726491.jpg


相关应用:


Phos-tag应用



相关产品:


 产品名称

 用  途

 Phos-tag™ Acrylamide

 分离:SDS - PAGE 分离不同磷酸化水平的蛋白

 SuperSep Phos-tag™

 分离:预制胶中含有 50 μM Phos-tag™ Acrylamide

 Phos-tag™ Biotin

 检测:代替 Western Blot 检测中的磷酸化抗体

 Phos-tag™ Agarose

 纯化:通用柱层析,纯化磷酸化蛋白

 Phos-tag™ Mass

 Analytical Kit

 分析:用于质谱 MALDI-TOF/MS 分析,提高磷酸化分子的检测灵敏度


phos-tag™ 由日本广岛大学研究生院医齿药学综合研究科医药分子功能科学研究室开发。


更多产品信息,请点击:http://phos-tag.jp



Phos-tag实验指导手册 第6版 2020.jpg


Phos-tag 第6版说明书




Phos-tag 系列产品封面图.jpg


Phos-tag系列 ver. 8




Q.     Phos-tag™ Mass 用于实验可以使用多少次?

A.     如果每次用量为 5 μL,至少可以使用 1000 次。



Q.     如何选择使用 Phos-tag™ MS-101L,Phos-tag™ MS-101H 和 Phos-tag™ MS-101N ?

A.     Phos-tag™ 101N 含有自然存在的 Zn,101L 与 101H 分别含有 Zn 的同位素 64Zn 和 68Zn。


     请参考以下建议:

     摸索条件时使用 101N,其中含有多种同位素,结果比较详细;

     鉴定磷酸基团是否存在,使用 101L 和 101H,这些试剂分别包含 64Zn 和 68Zn。使用这些试剂检测同一个样品时会产生不同的荷质比。



Q.     如果想测定经过 Phos-tag™ SDS-PAGE 分离得到的样品,是否必须要在凝胶消化之前去除 Phos-tag™?

A.     没有必要。SDS-PAGE 结束之后根据一般的凝胶消化方法进行操作即可。



Q.     能否用于 ESI 质谱?

A.     是的,可以使用。请参考下面的文献,这篇报道使用 Phos-tag™ MS-101N 进行 ESI-MS 分析。

      在实验过程中,使用了中性溶液,若为酸性溶液会导致 Phos-tag ™ 分离。


        【参考文献】 Anal. Chem. (2008), 80, 2531-2538 (MS-101N ESI-MS)


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References on Phos-tag™ Chemistry



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